GSM5426271: RB-C4-2-Veh rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RB1
Cell type
Cell type Class
Prostate
Cell type
C4-2
Tissue
Prostate
Cell Type
Epithelial
Disease
Prostate Cancer
Attributes by original data submitter
Sample
source_name
C4-2 cell
cell type
prostate cancer cell line
medium condition
In RPMI 1640 medium with 5% charcoal-stripped FBS (CSS)
cell line
C4-2
treatment
untreated
chip antibody
anti-RB (Cell Signaling)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were constructed using the SMARTer ThruPLEX DNA-Seq Prep Kit (Takara Bio USA).